Part:BBa_K1960000:Experience

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Applications of BBa_K1960000

We found that the concentration of AHL could have an effect on the expression of this device. In order to find out the relation, we decided to make a AHL gradient induction for this device. As shown in the graph, the fluorescence intensity data and time on the AHL of different concentrations show the the relationship of AHL to the work of this device.

Figure 1 The mean cyan fluorescence intensity in E.coli and its variety with time on the AHL of 1ng/μL, 10ng/μL, 20ng/μL and 0ng/μL.

AHL would influenced the expression of CFP directly. The higher the concentration of AHL is, the more CFP expressed in a range from 1ng/μL to 10ng/μL. In addition, the expression time of CFP was different. T-test showed that CFP in 10ng/μL and 20ng/μL group started to express in the 240th minute and it started to express in the 480th minute in 1ng/μL group.

Figure 2 The mean green fluorescence intensity in E.coli and its variety with time on the AHL of 1ng/μL, 10ng/μL, 20ng/μL and 0ng/μL.

The concentration of AHL didn’t influence the expression of GFP directly. The GFP in 10ng/μL and 20ng/μL group started to express in the 420th minute and it had no significant difference in expression level. The GFP didn’t express in 1ng/μL group.

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